The Teratogenicity of Cyclophosphamide on Skeletal System and Neural Tube of Fetal Mice

Cyclophosphamide (CP) as an alkylating agent which is used for treatment of cancer and to prevent rejection of tissue transplantation. Therefore, in this study, the teratogenic effects of CP were compared. This study was performed on 11 pregnant mice that were divided into two groups. Control group received normal saline and test group received CP (20 mg/kg) intraperitoneal at 10 day of gestation, respectively. Fetuses were th collected at 19 day of gestation and after determination of weight and length; they were stained by Alizarin th red Alcian blue method. Cleft palate, spina bifida and exencephaly incidence were 62.79%, 62.79% and 30.23% in fetuses of mice that received only CP, respectively. In addition, skeletal anomalies incidence including limbs, vertebrae and sternum defects were observed by CP. The mean of weight and length of animals' fetuses that received normal saline were significantly greater than those received CP.